Luminescence Image Analyzer
Cytiva A :ImageQuant 800 B :ImageQuant 800 Fluor
Installation date: March, 2022
A :Restricted to “chemiluminescence”
B : For “chemiluminescence”, “fluorescence”, “gel analysis”, and “OD (Optical Density Measurements)”
Features
- Common to “A” and “B”
It is a CCD-based imager detecting both chemiluminescence and visible spectrum.- Enables easy to perform high sensitivity detection of western blotting without the need of a darkroom.
- Pre-Capture :
- automatically calculates the exposure time of the region of interest in the pre-captured image.
- SNOW(Signal Noise Optimization Watch :
- reduces noise and avoids saturation of the image by multiple exposures and merging them into a single signal. Both weak and strong bands can be captured without saturation. (e.g. phosphorylation bands, close bands)
- Both chemiluminescence sample image and colorimetric marker image can be captured and automatically overlaid.
- Image formats: 16bit gray scale TIF (Raw data) and JPEG (finalized image if edited)
- ImageQuant TL (analysis software) is available.
- Restricted to “B”
- Fluorescent imaging (A maximum of three dyes can be analyzed at one time by “Auto” mode and “manual” mode.)
- Chemiluminescence imaging and fluorescence imaging can be combined. (e.g. First, capture the target protein band with chemiluminescence and then, capture the house keeping band with fluorescence, and merge them automatically.)
- Gel documentation such as capturing images of CBB stained gels (White Epi light source)
- OD (Optical Density) measurements (White Trans light source)
- Non-Parallax(NP)lens is available. NP lens eliminates penpheral shadows of well plates in chemiluminescence.
Rules
- You must receive a training session.
- “A” should be used for chemiluminescense image capture.
- Neither “A” nor “B” can be used for EtBr detection.
- When using SNOW, which takes longer, use “B” preferentially. (Please consult us when you make a reservation.)
There are restrictions on time of use: 1 hour
About training session
- We conduct training sessions as needed. Please contact us in advance to adjust the schedule.
- The training session takes approximately 1 hour.
- Please contact us after you have decided when you will start using the equipment.
- There is no need to bring a sample, but if you wish to bring one please let us know.
Specifications (Restricted to “B”)
Filter and LED
Band-pass filter allows light with particular wavelength to pass through.
